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European Journal of Dermatology

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Study of housekeeping gene expression in human keratinocytes using OLISA™, a long-oligonucleotide microarray and q RT-PCR Volume 16, numéro 2, March-April 2006

Auteurs
Laboratoires LVMH Recherche, 185 avenue de Verdun, 45804 St Jean de Braye Cedex, France, bioMérieux, 5 rue des Berges, 38000 Grenoble, France, INSERM U503 and IFR 128, Unite d’Immunologie et Allergologie Clinique, Centre Hospitalier Lyon-Sud, F-69495 Pierre-Benite Cedex, France, Authors with equal contribution

In recent years, applications of microarray platforms have been extended to different areas of research including cosmetic and pharmaceutical. Although microarray technology is still improving its sensitivity and flexibility, researchers often turn toward quantitative RT-PCR for data validation. Assessment of messenger RNA quantity by these methods is based on comparison with internal standard genes, mainly housekeeping genes, so called because their synthesis occurs normally at a constant level. However, numerous studies showed that expression of these genes could vary in given situations. Here, we report results on four housekeeping genes (GAPDH, β-2 microglobulin, S40 and S26 ribosomal sub-units) with constant expression levels established on OLISA™ microarray using different keratinocyte cultures. Moreover, qRT-PCR validation demonstrates that S26 ribosomal is a good housekeeping gene on keratinocytes and skin studies. Our data indicate that S26 gene can be routinely used to standardize results to investigate differentially expressed genes in a healthy human skin.