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 Printable version |
Interleukin-12 induces gene expression in interleukin-2 stimulated human T lymphocytes. |
European Cytokine Network. Volume 11, Number 4, 602-7, December 2000, Articles originaux
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 Free Article
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Author(s) : B. Li, D. Cao, H. Xu, J. Chang, G. Zhou, J. Tian, D. Li, J. Theze, C. Wu |
Summary : IL-12 is a critical immunoregulatory cytokine that promotes cell-mediated immune responses by inducing the differentiation of Th1 cells. To better clarify the molecular basis of IL-12 action, we compared the gene expression in human T lymphocytes activated by IL-2 and IL-12. mRNAs from T lymphocytes activated by either IL-2 alone or IL-2 plus IL-12 were transcribed into cDNAs. A differential mRNA display was conducted. As a result, differential display of five cDNA fragments was obtained. Sequence analysis suggests that they had high homology with recorded genes as found by a computer search against GenBank. Two full genes of the five fragments were cloned, which activation-induced C-type lectin and glucose transporter-like protein. Interestingly, these proteins were expressed in the T cells stimulated by IL-2 and IL-12, but not in the T cells stimulated by IL-2 alone. These results suggest that C-type lectin and glucose transporter-like protein may play an important role in the T lymphocyte activation induced by IL-12. |
Keywords : IL-12, IL-2, T lymphocytes, C-type lectin, glucose transporter-like protein. |
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